› 3tm4

X-ray diffraction
1.95Å resolution

Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine

Released:
DOI: 10.2210/pdb3tm4/pdb
PDB entry 3tm4 coloured by chain, front view.
PDB entry 3tm4 coloured by chain, side view.
PDB entry 3tm4 coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.
Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versées W
Nucleic Acids Res 40 5149-61 (2012)
PMID: 22362751

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(6) in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(6) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine(6)-N2)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 42.13 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U248 (Residues: 1-365; Coverage: 100%)
Gene names: PF1002, trm14
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 82.08Å b: 45.17Å c: 121.43Å
α: 90° β: 91.79° γ: 90°
R-values:
R R work R free
0.183 0.182 0.196
Expression system: Escherichia coli

Quick links

Citations

7 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
6 more

5 mentions without citation

Transfer RNA Modification Enzymes with a Thiouridine Synthetase, Methyltransferase and Pseudouridine Synthase (THUMP) Domain and the Nucleosides They Produce in tRNA.
Hori H. (2023)
4 more