› 3tma

X-ray diffraction
2.05Å resolution

Crystal structure of TrmN from Thermus thermophilus

Released:
DOI: 10.2210/pdb3tma/pdb
PDB entry 3tma coloured by chain, front view.
PDB entry 3tma coloured by chain, side view.
PDB entry 3tma coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.
Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versées W
Nucleic Acids Res 40 5149-61 (2012)
PMID: 22362751

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(6) in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(6) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-180836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine(6)-N2)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.08 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72IH5 (Residues: 1-335; Coverage: 100%)
Gene names: TT_C1157, trmN
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3221
Unit cell:
a: 65.86Å b: 65.86Å c: 144.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.154 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
6 more

6 mentions without citation

Transfer RNA Modification Enzymes with a Thiouridine Synthetase, Methyltransferase and Pseudouridine Synthase (THUMP) Domain and the Nucleosides They Produce in tRNA.
Hori H. (2023)
5 more