› 3tn7

X-ray diffraction
1.68Å resolution

Crystal structure of short-chain alcohol dehydrogenase from hyperthermophilic archaeon Thermococcus sibiricus complexed with 5-hydroxy-NADP

Released:
DOI: 10.2210/pdb3tn7/pdb
PDB entry 3tn7 coloured by chain, front view.
PDB entry 3tn7 coloured by chain, side view.
PDB entry 3tn7 coloured by chain, top view.
Source organism: Thermococcus sibiricus MM 739
Primary publication:
Structural insight into the molecular basis of polyextremophilicity of short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus.
Bezsudnova EY, Boyko KM, Polyakov KM, Dorovatovskiy PV, Stekhanova TN, Gumerov VM, Ravin NV, Skryabin KG, Kovalchuk MV, Popov VO
Biochimie 94 2628-38 (2012)
PMID: 22885278

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain alcohol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.73 KDa
Source organism: Thermococcus sibiricus MM 739
Expression system: Escherichia coli
UniProt:
  • Canonical: C6A190 (Residues: 1-234; Coverage: 100%)
Gene name: TSIB_0319
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NJP 2 x NJP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: C2
Unit cell:
a: 146.18Å b: 55.5Å c: 82.77Å
α: 90° β: 124.53° γ: 90°
R-values:
R R work R free
0.148 0.146 0.181
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Rigidity versus flexibility: the dilemma of understanding protein thermal stability.
Karshikoff et al. (2015)
2 more