› 3tnm

X-ray diffraction
1.85Å resolution

Crystal structure of A32 Fab, an ADCC mediating anti-HIV-1 antibody

Released:
DOI: 10.2210/pdb3tnm/pdb
PDB entry 3tnm coloured by chain, front view.
PDB entry 3tnm coloured by chain, side view.
PDB entry 3tnm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Diverse specificity and effector function among human antibodies to HIV-1 envelope glycoprotein epitopes exposed by CD4 binding.
Guan Y, Pazgier M, Sajadi MM, Kamin-Lewis R, Al-Darmarki S, Flinko R, Lovo E, Wu X, Robinson JE, Seaman MS, Fouts TR, Gallo RC, DeVico AL, Lewis GK
Proc Natl Acad Sci U S A 110 E69-78 (2013)
PMID: 23237851

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213405 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab heavy chain of human anti-HIV-1 Env antibody A32 Chains: A, H
Molecule details ›
Chains: A, H
Length: 231 amino acids
Theoretical weight: 24.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab light chain of human anti-HIV-1 Env antibody A32 Chains: B, L
Molecule details ›
Chains: B, L
Length: 216 amino acids
Theoretical weight: 22.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P1
Unit cell:
a: 40.78Å b: 71.316Å c: 77.673Å
α: 103.69° β: 104.02° γ: 105.4°
R-values:
R R work R free
0.19 0.187 0.232
Expression system: Homo sapiens

Quick links

Citations

23 review citations

Epitope specificity of human immunodeficiency virus-1 antibody dependent cellular cytotoxicity [ADCC] responses.
Pollara et al. (2013)
22 more

8 mentions without citation

Diverse specificity and effector function among human antibodies to HIV-1 envelope glycoprotein epitopes exposed by CD4 binding.
Guan et al. (2013)
7 more