› 3to3

X-ray diffraction
2.38Å resolution

Crystal Structure of Petrobactin Biosynthesis Protein AsbB from Bacillus anthracis str. Sterne

Released:
DOI: 10.2210/pdb3to3/pdb
Model geometry
Fit model/data
PDB entry 3to3 coloured by chain, front view.
PDB entry 3to3 coloured by chain, side view.
PDB entry 3to3 coloured by chain, top view.
Source organism: Bacillus anthracis str. Sterne
Primary publication:
Functional and structural analysis of the siderophore synthetase AsbB through reconstitution of the petrobactin biosynthetic pathway from Bacillus anthracis.
Nusca TD, Kim Y, Maltseva N, Lee JY, Eschenfeldt W, Stols L, Schofield MM, Scaglione JB, Dixon SD, Oves-Costales D, Challis GL, Hanna PC, Pfleger BF, Joachimiak A, Sherman DH
J Biol Chem 287 16058-72 (2012)
PMID: 22408253

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citryl-spermidine/3,4-dihydroxybenzoyl-citryl-spermidine:spermidine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 619 amino acids
Theoretical weight: 73.5 KDa
Source organism: Bacillus anthracis str. Sterne
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81RQ8 (Residues: 3-612; Coverage: 100%)
Gene names: GBAA_1982, asbB
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand EDO 3 x EDO
Ligand ATP 1 x ATP
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 64.259Å b: 155.923Å c: 156.244Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
3 more

2 mentions without citation

Functional and structural analysis of the siderophore synthetase AsbB through reconstitution of the petrobactin biosynthetic pathway from Bacillus anthracis.
Nusca et al. (2012)
1 more