› 3tol

X-ray diffraction
2Å resolution

Crystal structure of an engineered cytochrome cb562 that forms 1D, Zn-mediated coordination polymers

Released:
DOI: 10.2210/pdb3tol/pdb
PDB entry 3tol coloured by chain, front view.
PDB entry 3tol coloured by chain, side view.
PDB entry 3tol coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays.
Brodin JD, Ambroggio XI, Tang C, Parent KN, Baker TS, Tezcan FA
Nat Chem 4 375-82 (2012)
PMID: 22522257

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

3 bound ligands:
Ligand HEM 4 x HEM
Ligand ZN 4 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 56.546Å b: 69.506Å c: 126.567Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.19 0.245
Expression system: Escherichia coli

Quick links

Citations

47 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
46 more

4 mentions without citation

Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays.
Brodin et al. (2012)
3 more