› 3tpy

X-ray diffraction
1.75Å resolution

Crystal structure of M-PMV dUTPase with a mixed population of substrate (dUPNPP) and post-inversion product (dUMP) in the active sites

Released:
DOI: 10.2210/pdb3tpy/pdb
PDB entry 3tpy coloured by chain, front view.
PDB entry 3tpy coloured by chain, side view.
PDB entry 3tpy coloured by chain, top view.
Source organism: Mason-Pfizer monkey virus
Entry authors: Barabas O, Nemeth V, Vertessy BG

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleocapsid protein-dUTPase Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 16.16 KDa
Source organism: Mason-Pfizer monkey virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07570 (Residues: 608-759; Coverage: 17%)
Gene name: gag-pro
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand TRS 1 x TRS
Ligand DUP 1 x DUP
Ligand UMP 1 x UMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13, ESRF BEAMLINE ID14-4
Spacegroup: P63
Unit cell:
a: 60.559Å b: 60.559Å c: 63.635Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.152 0.186
Expression system: Escherichia coli BL21(DE3)

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