› 3tqo

X-ray diffraction
2.3Å resolution

Structure of the cysteinyl-tRNA synthetase (cysS) from Coxiella burnetii.

Released:
DOI: 10.2210/pdb3tqo/pdb
PDB entry 3tqo coloured by chain, front view.
PDB entry 3tqo coloured by chain, side view.
PDB entry 3tqo coloured by chain, top view.
Source organism: Coxiella burnetii
Primary publication:
Structural genomics for drug design against the pathogen Coxiella burnetii.
Franklin MC, Cheung J, Rudolph MJ, Burshteyn F, Cassidy M, Gary E, Hillerich B, Yao ZK, Carlier PR, Totrov M, Love JD
Proteins 83 2124-36 (2015)
PMID: 26033498

Function and Biology Details

Reaction catalysed: 
ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 53.77 KDa
Source organism: Coxiella burnetii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83BL7 (Residues: 1-460; Coverage: 100%)
Gene names: CBU_1487, cysS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P41212
Unit cell:
a: 97.632Å b: 97.632Å c: 108.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.26
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology.
Martins et al. (2015)
3 more

2 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
1 more