› 3tr6

X-ray diffraction
2.7Å resolution

Structure of a O-methyltransferase from Coxiella burnetii

Released:
DOI: 10.2210/pdb3tr6/pdb
PDB entry 3tr6 coloured by chain, front view.
PDB entry 3tr6 coloured by chain, side view.
PDB entry 3tr6 coloured by chain, top view.
Source organism: Coxiella burnetii
Primary publication:
Structural genomics for drug design against the pathogen Coxiella burnetii.
Franklin MC, Cheung J, Rudolph MJ, Burshteyn F, Cassidy M, Gary E, Hillerich B, Yao ZK, Carlier PR, Totrov M, Love JD
Proteins 83 2124-36 (2015)
PMID: 26033498

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.4 KDa
Source organism: Coxiella burnetii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83D22 (Residues: 1-222; Coverage: 100%)
Gene name: CBU_0924
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand SAH 1 x SAH
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6122
Unit cell:
a: 83.686Å b: 83.686Å c: 212.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology.
Martins et al. (2015)
3 more

3 mentions without citation

Structure and Biophysical Characterization of the S-Adenosylmethionine-dependent O-Methyltransferase PaMTH1, a Putative Enzyme Accumulating during Senescence of Podospora anserina.
Chatterjee et al. (2015)
2 more