› 3tsg

X-ray diffraction
1.9Å resolution

Crystal structure of GES-14

Released:
DOI: 10.2210/pdb3tsg/pdb
PDB entry 3tsg coloured by chain, front view.
PDB entry 3tsg coloured by chain, side view.
PDB entry 3tsg coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Kinetic and crystallographic studies of extended-spectrum GES-11, GES-12, and GES-14 β-lactamases.
Delbrück H, Bogaerts P, Kupper MB, Rezende de Castro R, Bennink S, Glupczynski Y, Galleni M, Hoffmann KM, Bebrone C
Antimicrob Agents Chemother 56 5618-25 (2012)
PMID: 22908160

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.24 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D3X610 (Residues: 1-287; Coverage: 100%)
Gene names: ESBL, blaGES-14
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand IOD 55 x IOD
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Unit cell:
a: 70.745Å b: 84.098Å c: 85.026Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.205
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii: Mechanisms and epidemiology.
Potron et al. (2015)
1 more

2 mentions without citation

Kinetic and crystallographic studies of extended-spectrum GES-11, GES-12, and GES-14 β-lactamases.
Delbrück et al. (2012)
1 more