› 3ttq

X-ray diffraction
1.9Å resolution

Crystal structure of Leuconostoc mesenteroides NRRL B-1299 N-terminally truncated dextransucrase DSR-E in orthorhombic apo-form at 1.9 angstrom resolution

Released:
DOI: 10.2210/pdb3ttq/pdb
Model geometry
Fit model/data
PDB entry 3ttq coloured by chain, front view.
PDB entry 3ttq coloured by chain, side view.
PDB entry 3ttq coloured by chain, top view.
Source organism: Leuconostoc mesenteroides
Primary publication:
Functional and structural characterization of α-(1->2) branching sucrase derived from DSR-E glucansucrase.
Brison Y, Pijning T, Malbert Y, Fabre É, Mourey L, Morel S, Potocki-Véronèse G, Monsan P, Tranier S, Remaud-Siméon M, Dijkstra BW
J Biol Chem 287 7915-24 (2012)
PMID: 22262856

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dextransucrase Chain: A
Molecule details ›
Chain: A
Length: 1108 amino acids
Theoretical weight: 123.47 KDa
Source organism: Leuconostoc mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8G9Q2 (Residues: 1759-2835; Coverage: 39%)
Gene name: dsrE
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand CA 1 x CA
Ligand GOL 23 x GOL
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 68.18Å b: 100.24Å c: 187.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.198
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.
Schmid et al. (2015)
8 more

11 mentions without citation

Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes.
Meng et al. (2016)
10 more