› 3ttx

X-ray diffraction
1.74Å resolution

Structure of the F413K variant of E. coli KatE

Released:
DOI: 10.2210/pdb3ttx/pdb
PDB entry 3ttx coloured by chain, front view.
PDB entry 3ttx coloured by chain, side view.
PDB entry 3ttx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mutation of Phe413 to Tyr in catalase KatE from Escherichia coli leads to side chain damage and main chain cleavage.
Jha V, Donald LJ, Loewen PC
Arch Biochem Biophys 525 207-14 (2012)
PMID: 22172685

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase HPII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 753 amino acids
Theoretical weight: 84.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P21179 (Residues: 1-753; Coverage: 100%)
Gene names: JW1721, b1732, katE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
No bound ligands
1 modified residue:
Ligand OCS 4 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 93.5Å b: 132.96Å c: 122.03Å
α: 90° β: 109.69° γ: 90°
R-values:
R R work R free
0.148 0.146 0.185
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Monofunctional Heme-Catalases.
Hansberg W. (2022)
1 other article