› 3tu0

X-ray diffraction
2.99Å resolution

Crystal structure of T355V, S354A, K288A LeuT mutant in complex with alanine and sodium

Released:
DOI: 10.2210/pdb3tu0/pdb
PDB entry 3tu0 coloured by chain, front view.
PDB entry 3tu0 coloured by chain, side view.
PDB entry 3tu0 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.
Krishnamurthy H, Gouaux E
Nature 481 469-74 (2012)
PMID: 22230955

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand ALA 1 x ALA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 89.331Å b: 87.346Å c: 81.687Å
α: 90° β: 95.72° γ: 90°
R-values:
R R work R free
0.221 0.218 0.288
Expression system: Escherichia coli

Quick links

Citations

77 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
76 more

2 mentions without citation

X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.
Krishnamurthy et al. (2012)
1 more