› 3tu3

X-ray diffraction
1.92Å resolution

1.92 Angstrom resolution crystal structure of the full-length SpcU in complex with full-length ExoU from the type III secretion system of Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3tu3/pdb
PDB entry 3tu3 coloured by chain, front view.
PDB entry 3tu3 coloured by chain, side view.
PDB entry 3tu3 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of the type III secretion effector protein ExoU in complex with its chaperone SpcU.
Halavaty AS, Borek D, Tyson GH, Veesenmeyer JL, Shuvalova L, Minasov G, Otwinowski Z, Hauser AR, Anderson WF
PLoS One 7 e49388 (2012)
PMID: 23166655

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128522 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SpcU Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 17.68 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66100 (Residues: 1-137; Coverage: 100%)
Gene names: EXA77, EXC3, GNQ48_30365, RS15, spcU
Structure domains: Yope Regulator; Chain: A,
PNPLA domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 711 amino acids
Theoretical weight: 76.76 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34208 (Residues: 1-687; Coverage: 100%)
Gene names: EXC2, RS14, exoU, pepA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D, APS BEAMLINE 19-ID, APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 154.14Å b: 52.583Å c: 119.539Å
α: 90° β: 126.59° γ: 90°
R-values:
R R work R free
0.193 0.191 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Assembly and structure of the T3SS.
Burkinshaw et al. (2014)
5 more

19 mentions without citation

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.
Flores-Díaz et al. (2016)
18 more