› 3tvd

X-ray diffraction
2.99Å resolution

Crystal Structure of Mouse RhoA-GTP complex

Released:
DOI: 10.2210/pdb3tvd/pdb
PDB entry 3tvd coloured by chain, front view.
PDB entry 3tvd coloured by chain, side view.
PDB entry 3tvd coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of mouse RhoA:GTPγS complex in a centered lattice.
Jobichen C, Pal K, Swaminathan K
J Struct Funct Genomics 13 241-5 (2012)
PMID: 23001747

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 21.81 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61589 (Residues: 1-193; Coverage: 100%)
Gene names: Arha, Arha2, Rhoa
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GSP 2 x GSP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C222
Unit cell:
a: 80.813Å b: 122.652Å c: 91.276Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.223 0.282
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Signaling networks of Rho GTPases in cell motility.
Hanna et al. (2013)
1 other article

6 mentions without citation

Structural Dynamics in Ras and Related Proteins upon Nucleotide Switching.
Harrison et al. (2016)
5 more