› 3tvy

X-ray diffraction
2Å resolution

Structural Analysis of Adhesive Tip pilin, GBS104 from Group B Streptococcus agalactiae

Released:
DOI: 10.2210/pdb3tvy/pdb
PDB entry 3tvy coloured by chain, front view.
PDB entry 3tvy coloured by chain, side view.
PDB entry 3tvy coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup V
Primary publication:
Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS pili assembly and host interactions.
Krishnan V, Dwivedi P, Kim BJ, Samal A, Macon K, Ma X, Mishra A, Doran KS, Ton-That H, Narayana SV
Acta Crystallogr D Biol Crystallogr 69 1073-89 (2013)
PMID: 23695252

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VWFA domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 41.98 KDa
Source organism: Streptococcus agalactiae serogroup V
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8E0S5 (Residues: 212-584; Coverage: 43%)
Gene name: SAG0649
Sequence domains: von Willebrand factor type A domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EPE 1 x EPE
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 74.749Å b: 101.29Å c: 52.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.251
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Pilins in gram-positive bacteria: A structural perspective.
Krishnan V. (2015)
3 more

2 mentions without citation

Pilus biogenesis of Gram-positive bacteria: Roles of sortases and implications for assembly.
Khare et al. (2017)
1 more