› 3tw8

X-ray diffraction
2.1Å resolution

GEF domain of DENND 1B in complex with Rab GTPase Rab35

Released:
DOI: 10.2210/pdb3tw8/pdb
PDB entry 3tw8 coloured by chain, front view.
PDB entry 3tw8 coloured by chain, side view.
PDB entry 3tw8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights regarding guanine nucleotide exchange from the structure of a DENN-domain protein complexed with its Rab GTPase substrate.
Wu X, Bradley MJ, Cai Y, Kümmel D, De La Cruz EM, Barr FA, Reinisch KM
Proc Natl Acad Sci U S A 108 18672-7 (2011)
PMID: 22065758

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172246 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DENN domain-containing protein 1B Chains: A, C
Molecule details ›
Chains: A, C
Length: 391 amino acids
Theoretical weight: 44.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P3S1 (Residues: 1-410; Coverage: 50%)
Gene names: C1orf218, DENND1B, FAM31B
Sequence domains:
Structure domains:
Ras-related protein Rab-35 Chains: B, D
Molecule details ›
Chains: B, D
Length: 181 amino acids
Theoretical weight: 20.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15286 (Residues: 1-180; Coverage: 90%)
Gene names: RAB1C, RAB35, RAY
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 122.588Å b: 56.563Å c: 175.305Å
α: 90° β: 95.63° γ: 90°
R-values:
R R work R free
0.23 0.227 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Rab proteins and the compartmentalization of the endosomal system.
Wandinger-Ness et al. (2014)
14 more

12 mentions without citation

Molecular control of Rab activity by GEFs, GAPs and GDI.
Müller et al. (2018)
11 more