Function and Biology Details
Reactions catalysed:
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
Formamidopyrimidine-DNA glycosylase and DNA (preferred)
PDBe Complex ID:
PDB-CPX-116948 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 34.69 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 310 amino acids
Theoretical weight: 34.69 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
O80358 (Residues: 1-304; Coverage: 78%)
Sequence domains:
- Formamidopyrimidine-DNA glycosylase N-terminal domain
- Formamidopyrimidine-DNA glycosylase H2TH domain
- Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 5.0.3
Spacegroup:
P212121
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
