› 3u01

X-ray diffraction
1.12Å resolution

Crystal structure of onconase double mutant C30A/C75A at 1.12 A resolution

Released:
DOI: 10.2210/pdb3u01/pdb
PDB entry 3u01 coloured by chain, front view.
PDB entry 3u01 coloured by chain, side view.
PDB entry 3u01 coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
Investigating the effects of double mutation C30A/C75A on onconase structure: Studies at atomic resolution.
Kurpiewska K, Torrent G, Ribó M, Loch JI, Vilanova M, Lewiński K
Biopolymers 101 454-60 (2014)
PMID: 23996687

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein P-30 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.78 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22069 (Residues: 1-104; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand ACT 1 x ACT
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P212121
Unit cell:
a: 32.288Å b: 38.664Å c: 69.514Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.15
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Solution structure and base specificity of cytotoxic RC-RNase 2 from Rana catesbeiana.
Hsu et al. (2015)