› 3u09

X-ray diffraction
1.75Å resolution

Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92D) from Vibrio cholerae

Released:
DOI: 10.2210/pdb3u09/pdb
PDB entry 3u09 coloured by chain, front view.
PDB entry 3u09 coloured by chain, side view.
PDB entry 3u09 coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
Dissecting the Structural Elements for the Activation of β-Ketoacyl-(Acyl Carrier Protein) Reductase from Vibrio cholerae.
Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W
J Bacteriol 198 463-76 (2016)
PMID: 26553852

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase FabG Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 26.42 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KQH7 (Residues: 1-244; Coverage: 100%)
Gene names: VC_2021, fabG
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand UNX 10 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P62
Unit cell:
a: 63.574Å b: 63.574Å c: 191.059Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.187
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Indole Biodegradation in Acinetobacter sp. Strain O153: Genetic and Biochemical Characterization.
Sadauskas et al. (2017)
6 more

1 mention without citation

Dissecting the Structural Elements for the Activation of β-Ketoacyl-(Acyl Carrier Protein) Reductase from Vibrio cholerae.
Hou et al. (2016)