› 3u0c

X-ray diffraction
2.05Å resolution

Crystal structure of N-terminal region of Type III Secretion First Translocator IpaB (residues 74-224)

Released:
DOI: 10.2210/pdb3u0c/pdb
PDB entry 3u0c coloured by chain, front view.
PDB entry 3u0c coloured by chain, side view.
PDB entry 3u0c coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
The structures of coiled-coil domains from type III secretion system translocators reveal homology to pore-forming toxins.
Barta ML, Dickenson NE, Patil M, Keightley A, Wyckoff GJ, Picking WD, Picking WL, Geisbrecht BV
J Mol Biol 417 395-405 (2012)
PMID: 22321794

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system translocon protein SctE Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.46 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18011 (Residues: 28-226; Coverage: 34%)
Gene names: CP0128, ipaB, sctE
Sequence domains: Type III cell invasion protein SipB
Structure domains: Nucleotidyltransferases domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 52.419Å b: 28.379Å c: 104.806Å
α: 90° β: 95.99° γ: 90°
R-values:
R R work R free
0.248 0.245 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
17 more

6 mentions without citation

The structures of coiled-coil domains from type III secretion system translocators reveal homology to pore-forming toxins.
Barta et al. (2012)
5 more