› 3u12

X-ray diffraction
2.08Å resolution

The pleckstrin homology (PH) domain of USP37

Released:
DOI: 10.2210/pdb3u12/pdb
PDB entry 3u12 coloured by chain, front view.
PDB entry 3u12 coloured by chain, side view.
PDB entry 3u12 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Dong A, Nair UB, Wernimont A, Walker JR, Weigelt J, Bountra C, Edwards AM, Arrowsmith CH, Dhe-Paganon S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 37 Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86T82 (Residues: 4-125; Coverage: 13%)
Gene names: KIAA1594, USP37
Sequence domains: N-terminal of ubiquitin carboxyl-terminal hydrolase 37
Structure domains: Ubiquitin carboxyl-terminal hydrolase 26/29/37, pleckstrin homology-like domain

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand ACT 2 x ACT
Ligand UNX 29 x UNX
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P6122
Unit cell:
a: 64.083Å b: 64.083Å c: 218.814Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.232
Expression system: Escherichia coli BL21(DE3)

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