› 3u28

X-ray diffraction
1.9Å resolution

Crystal structure of a Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3u28/pdb
Model geometry
Fit model/data
PDB entry 3u28 coloured by chain, front view.
PDB entry 3u28 coloured by chain, side view.
PDB entry 3u28 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Reconstitution and structural analysis of the yeast box H/ACA RNA-guided pseudouridine synthase.
Li S, Duan J, Li D, Yang B, Dong M, Ye K
Genes Dev 25 2409-21 (2011)
PMID: 22085967

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151151 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H/ACA ribonucleoprotein complex subunit CBF5 Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 44.98 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33322 (Residues: 3-394; Coverage: 81%)
Gene names: CBF5, L9470.11, YLR175W
Sequence domains:
Structure domains:
H/ACA ribonucleoprotein complex subunit NOP10 Chain: B
Molecule details ›
Chain: B
Length: 58 amino acids
Theoretical weight: 6.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6Q547 (Residues: 1-58; Coverage: 100%)
Gene names: NOP10, YHR072W-A
Sequence domains: Nucleolar RNA-binding protein, Nop10p family
Structure domains: Rhinovirus 14, subunit 4
H/ACA ribonucleoprotein complex subunit GAR1 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P212121
Unit cell:
a: 54.413Å b: 59.386Å c: 169.212Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.233
Expression system: Escherichia coli

Quick links

Citations

18 review citations

The emerging landscape of small nucleolar RNAs in cell biology.
Dupuis-Sandoval et al. (2015)
17 more

11 mentions without citation

Dyskerin: an essential pseudouridine synthase with multifaceted roles in ribosome biogenesis, splicing, and telomere maintenance.
Garus et al. (2021)
10 more