› 3u2m

X-ray diffraction
2Å resolution

Crystal structure of human ALR mutant C142/145S

Released:
DOI: 10.2210/pdb3u2m/pdb
PDB entry 3u2m coloured by chain, front view.
PDB entry 3u2m coloured by chain, side view.
PDB entry 3u2m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An electron-transfer path through an extended disulfide relay system: the case of the redox protein ALR.
Banci L, Bertini I, Calderone V, Cefaro C, Ciofi-Baffoni S, Gallo A, Tokatlidis K
J Am Chem Soc 134 1442-5 (2012)
PMID: 22224850

Function and Biology Details

Reaction catalysed: 
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-linked sulfhydryl oxidase ALR Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55789 (Residues: 91-205; Coverage: 56%)
Gene names: ALR, GFER, HERV1, HPO
Sequence domains: Erv1 / Alr family
Structure domains: ERV/ALR sulfhydryl oxidase domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: C2221
Unit cell:
a: 50.4Å b: 76.88Å c: 62.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.23 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Mitochondrial Machineries for Protein Import and Assembly.
Wiedemann et al. (2017)
8 more