› 3u33

X-ray diffraction
2.8Å resolution

Crystal Structure of the E. coli adaptive response protein AidB in the space group P3(2)

Released:
DOI: 10.2210/pdb3u33/pdb
PDB entry 3u33 coloured by chain, front view.
PDB entry 3u33 coloured by chain, side view.
PDB entry 3u33 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Flavin-induced oligomerization in Escherichia coli adaptive response protein AidB.
Hamill MJ, Jost M, Wong C, Elliott SJ, Drennan CL
Biochemistry 50 10159-69 (2011)
PMID: 22004173

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative acyl-CoA dehydrogenase AidB Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 541 amino acids
Theoretical weight: 60.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33224 (Residues: 1-541; Coverage: 100%)
Gene names: JW5867, aidB, b4187
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 12 x FAD
Ligand CL 12 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P32
Unit cell:
a: 179.72Å b: 179.72Å c: 204.237Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Co-factor binding confers substrate specificity to xylose reductase from Debaryomyces hansenii.
Biswas et al. (2012)
4 more

1 mention without citation

Electrochemical characterization of Escherichia coli adaptive response protein AidB.
Hamill et al. (2012)