› 3u3j

X-ray diffraction
2.7Å resolution

Crystal structure of hSULT1A1 bound to PAP

Released:
DOI: 10.2210/pdb3u3j/pdb
PDB entry 3u3j coloured by chain, front view.
PDB entry 3u3j coloured by chain, side view.
PDB entry 3u3j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular basis for the broad substrate specificity of human sulfotransferase 1A1.
Berger I, Guttman C, Amar D, Zarivach R, Aharoni A
PLoS One 6 e26794 (2011)
PMID: 22069470

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 36.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50225 (Residues: 1-294; Coverage: 100%)
Gene names: OK/SW-cl.88, STP, STP1, SULT1A1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand A3P 2 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 72.097Å b: 79.422Å c: 121.807Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.219 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

The Regulation of Steroid Action by Sulfation and Desulfation.
Mueller et al. (2015)
10 more

6 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
5 more