› 3u55

X-ray diffraction
1.9Å resolution

Crystal structure (Type-2) of SAICAR synthetase from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb3u55/pdb
PDB entry 3u55 coloured by chain, front view.
PDB entry 3u55 coloured by chain, side view.
PDB entry 3u55 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structure of SAICAR synthetase from Pyrococcus horikoshii OT3: insights into thermal stability.
Manjunath K, Kanaujia SP, Kanagaraj S, Jeyakanthan J, Sekar K
Int J Biol Macromol 53 7-19 (2013)
PMID: 23137517

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.48 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57978 (Residues: 1-238; Coverage: 100%)
Gene names: PH0239, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 44.1Å b: 155.39Å c: 78.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.229
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Structures of SAICAR synthetase (PurC) from Streptococcus pneumoniae with ADP, Mg2+, AIR and Asp.
Wolf et al. (2014)
4 more