› 3u56

X-ray diffraction
2.1Å resolution

Crystal structure of mutant ribosomal protein T217V TthL1 in complex with 80nt 23S RNA from Thermus thermophilus

Released:
DOI: 10.2210/pdb3u56/pdb
PDB entry 3u56 coloured by chain, front view.
PDB entry 3u56 coloured by chain, side view.
PDB entry 3u56 coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Gabdulkhakov AG, Nevskaya NA, NIkonov SV

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150938 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein uL1 Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.87 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27150 (Residues: 1-229; Coverage: 100%)
Gene names: rpl1, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Structure domains:
RNA (80-MER) Chain: B
Molecule details ›
Chain: B
Length: 80 nucleotides
Theoretical weight: 25.97 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
Sequence domains: Bacterial large subunit ribosomal RNA

Ligands and Environments

2 bound ligands:
Ligand MG 11 x MG
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 73.69Å b: 75.41Å c: 85.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.238
Expression system: Escherichia coli BL21(DE3)

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