› 3u5m

X-ray diffraction
3.08Å resolution

Crystal structure of TRIM33 PHD-Bromo in the free state

Released:
DOI: 10.2210/pdb3u5m/pdb
PDB entry 3u5m coloured by chain, front view.
PDB entry 3u5m coloured by chain, side view.
PDB entry 3u5m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A poised chromatin platform for TGF-β access to master regulators.
Xi Q, Wang Z, Zaromytidou AI, Zhang XH, Chow-Tsang LF, Liu JX, Kim H, Barlas A, Manova-Todorova K, Kaartinen V, Studer L, Mark W, Patel DJ, Massagué J
Cell 147 1511-24 (2011)
PMID: 22196728

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase TRIM33 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 207 amino acids
Theoretical weight: 23.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UPN9 (Residues: 882-1087; Coverage: 18%)
Gene names: KIAA1113, RFG7, TIF1G, TRIM33
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 24 x ZN
Ligand CA 12 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 79.527Å b: 79.781Å c: 134.16Å
α: 89.9° β: 89.96° γ: 59.97°
R-values:
R R work R free
0.211 0.207 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

82 review citations

Molecular mechanisms of epithelial-mesenchymal transition.
Lamouille et al. (2014)
81 more

3 mentions without citation

A poised chromatin platform for TGF-β access to master regulators.
Xi et al. (2011)
2 more