3u5z

X-ray diffraction
3.5Å resolution

Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog

Released:
Source organism: Tequatrovirus T4
Primary publication:
How a DNA polymerase clamp loader opens a sliding clamp.
Science 334 1675-80 (2011)
PMID: 22194570

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116959 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Sliding-clamp-loader large subunit Chains: B, C, D, E, L, M, N, O
Molecule details ›
Chains: B, C, D, E, L, M, N, O
Length: 324 amino acids
Theoretical weight: 36.19 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04526 (Residues: 1-319; Coverage: 100%)
Gene name: 44
Sequence domains:
Structure domains:
Sliding-clamp-loader small subunit Chains: A, K
Molecule details ›
Chains: A, K
Length: 199 amino acids
Theoretical weight: 22.94 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04527 (Residues: 2-187; Coverage: 100%)
Gene name: 62
Sequence domains: Bacteriophage clamp loader A subunit
Structure domains:
Sliding clamp Chains: F, G, H, P, Q, R
Molecule details ›
Chains: F, G, H, P, Q, R
Length: 228 amino acids
Theoretical weight: 25.16 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04525 (Residues: 1-228; Coverage: 100%)
Gene name: 45
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
Template DNA strand Chains: I, S
Molecule details ›
Chains: I, S
Length: 30 nucleotides
Theoretical weight: 9.17 KDa
Primer DNA strand Chains: J, T
Molecule details ›
Chains: J, T
Length: 20 nucleotides
Theoretical weight: 6.14 KDa

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 95.702Å b: 239.177Å c: 247.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.279
Expression system: Escherichia coli