› 3u7v

X-ray diffraction
1.8Å resolution

The structure of a putative Beta-galactosidase from Caulobacter crescentus CB15.

Released:
DOI: 10.2210/pdb3u7v/pdb
PDB entry 3u7v coloured by chain, front view.
PDB entry 3u7v coloured by chain, side view.
PDB entry 3u7v coloured by chain, top view.
Source organism: Caulobacter vibrioides NA1000
Entry authors: Cuff ME, Tesar C, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chain: A
Molecule details ›
Chain: A
Length: 552 amino acids
Theoretical weight: 61.11 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3C5N2 (Residues: 1-549; Coverage: 100%)
Gene name: CCNA_00830
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACY 2 x ACY
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4
Unit cell:
a: 148.306Å b: 148.306Å c: 53.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.215
Expression system: Escherichia coli BL21(DE3)

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