› 3u8i

X-ray diffraction
1.1Å resolution

Functionally selective inhibition of Group IIA phospholipase A2 reveals a role for vimentin in regulating arachidonic acid metabolism

Released:
DOI: 10.2210/pdb3u8i/pdb
PDB entry 3u8i coloured by chain, front view.
PDB entry 3u8i coloured by chain, side view.
PDB entry 3u8i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Selective inhibition of human group IIA-secreted phospholipase A2 (hGIIA) signaling reveals arachidonic acid metabolism is associated with colocalization of hGIIA to vimentin in rheumatoid synoviocytes.
Lee LK, Bryant KJ, Bouveret R, Lei PW, Duff AP, Harrop SJ, Huang EP, Harvey RP, Gelb MH, Gray PP, Curmi PM, Cunningham AM, Church WB, Scott KF
J Biol Chem 288 15269-79 (2013)
PMID: 23482564

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, membrane associated Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 14.14 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P14555 (Residues: 21-144; Coverage: 100%)
Gene names: PLA2B, PLA2G2A, PLA2L, RASF-A
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand CL 4 x CL
Ligand NA 4 x NA
1 modified residue:
Ligand OLD 2 x OLD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P41
Unit cell:
a: 70.426Å b: 70.426Å c: 47.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.186
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

Human Group IIA Phospholipase A2-Three Decades on from Its Discovery.
Scott et al. (2021)
2 more

5 mentions without citation

Structural and Functional Aspects of Targeting the Secreted Human Group IIA Phospholipase A2.
Kim et al. (2020)
4 more