› 3u9l

X-ray diffraction
2.1Å resolution

The crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) from Sinorhizobium meliloti

Released:
DOI: 10.2210/pdb3u9l/pdb
PDB entry 3u9l coloured by chain, front view.
PDB entry 3u9l coloured by chain, side view.
PDB entry 3u9l coloured by chain, top view.
Source organism: Sinorhizobium meliloti
Entry authors: Zhang Z, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 35.48 KDa
Source organism: Sinorhizobium meliloti
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92W71 (Residues: 1-300; Coverage: 100%)
Gene name: SM_b20492
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I222
Unit cell:
a: 58.632Å b: 106.123Å c: 128.796Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.236
Expression system: Escherichia coli BL21(DE3)

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