› 3ud9

X-ray diffraction
2.34Å resolution

Crystal Structure Analysis of FGF1-Disaccharide(NI23) complex

Released:
DOI: 10.2210/pdb3ud9/pdb
PDB entry 3ud9 coloured by chain, front view.
PDB entry 3ud9 coloured by chain, side view.
PDB entry 3ud9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Divergent synthesis of 48 heparan sulfate-based disaccharides and probing the specific sugar-fibroblast growth factor-1 interaction.
Hu YP, Zhong YQ, Chen ZG, Chen CY, Shi Z, Zulueta MM, Ku CC, Lee PY, Wang CC, Hung SC
J Am Chem Soc 134 20722-7 (2012)
PMID: 23240683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Fibroblast growth factor 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 141 amino acids
Theoretical weight: 15.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05230 (Residues: 16-155; Coverage: 90%)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

Carbohydrate polymer : NEW Components: IDY, SGN
Carbohydrate polymer
1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 113.574Å b: 46.93Å c: 96.708Å
α: 90° β: 125.71° γ: 90°
R-values:
R R work R free
0.216 0.212 0.252
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Heparan sulfate and heparin interactions with proteins.
Meneghetti et al. (2015)
10 more

1 mention without citation

Computational drill down on FGF1-heparin interactions through methodological evaluation.
Babik et al. (2017)