› 3ufg

X-ray diffraction
2.55Å resolution

The crystal structure of glycyl-tRNA synthetase subunit alpha from Campylobacter jejuni subsp. jejuni NCTC in complex with ATP

Released:
DOI: 10.2210/pdb3ufg/pdb
PDB entry 3ufg coloured by chain, front view.
PDB entry 3ufg coloured by chain, side view.
PDB entry 3ufg coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Tan K, Zhou M, Peterson S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine--tRNA ligase alpha subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 36.25 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PPK3 (Residues: 1-287; Coverage: 100%)
Gene names: Cj0704, glyQ
Sequence domains: Glycyl-tRNA synthetase alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand LEU 1 x LEU
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 114.58Å b: 114.58Å c: 157.707Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.174 0.255
Expression system: Escherichia coli

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