› 3ugh

X-ray diffraction
2.9Å resolution

Crystal structure of a 6-SST/6-SFT from Pachysandra terminalis in complex with 6-kestose

Released:
DOI: 10.2210/pdb3ugh/pdb
PDB entry 3ugh coloured by chain, front view.
PDB entry 3ugh coloured by chain, side view.
PDB entry 3ugh coloured by chain, top view.
Source organism: Pachysandra terminalis
Primary publication:
Crystal structure of 6-SST/6-SFT from Pachysandra terminalis, a plant fructan biosynthesizing enzyme in complex with its acceptor substrate 6-kestose.
Lammens W, Le Roy K, Yuan S, Vergauwen R, Rabijns A, Van Laere A, Strelkov SV, Van den Ende W
Plant J 70 205-19 (2012)
PMID: 22098191

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
beta-fructofuranosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 546 amino acids
Theoretical weight: 61.22 KDa
Source organism: Pachysandra terminalis
UniProt:
  • Canonical: E3PQS3 (Residues: 110-655; Coverage: 83%)
Gene names: 6-SFT, 6-SST
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA, XYS, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 88.291Å b: 123.894Å c: 146.699Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.24

Quick links

Citations

4 review citations

Review of Levan polysaccharide: From a century of past experiences to future prospects.
Öner et al. (2016)
3 more

1 mention without citation

In Silico insights on enhancing thermostability and activity of a plant Fructosyltransferase from Pachysandra terminalis via introduction of disulfide bridges.
Ortiz et al. (2019)