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› 3ujz

X-ray diffraction
2.5Å resolution

Crystal structure of enterohemorrhagic E. coli StcE

Released:
DOI: 10.2210/pdb3ujz/pdb
Model geometry
Fit model/data
PDB entry 3ujz coloured by chain, front view.
PDB entry 3ujz coloured by chain, side view.
PDB entry 3ujz coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Structural insight into the bacterial mucinase StcE essential to adhesion and immune evasion during enterohemorrhagic E. coli infection.
Yu AC, Worrall LJ, Strynadka NC
Structure 20 707-17 (2012)
PMID: 22483117

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-131381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metalloprotease StcE Chain: A
Molecule details ›
Chain: A
Length: 869 amino acids
Theoretical weight: 96.9 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: O82882 (Residues: 36-898; Coverage: 100%)
Gene names: ECO57PM83, L7031, stcE, tagA
Sequence domains:
Structure domains: Gamma-B Crystallin; domain 1

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 17 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 76.53Å b: 186Å c: 188.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.252
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Colonization resistance and microbial ecophysiology: using gnotobiotic mouse models and single-cell technology to explore the intestinal jungle.
Stecher et al. (2013)
7 more

4 mentions without citation

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
3 more