› 3ukf

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF UDP-galactopyranose mutase from Aspergillus fumigatus in complex with UDPgalp (reduced)

Released:
DOI: 10.2210/pdb3ukf/pdb
PDB entry 3ukf coloured by chain, front view.
PDB entry 3ukf coloured by chain, side view.
PDB entry 3ukf coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Structural insight into the unique substrate binding mechanism and flavin redox state of UDP-galactopyranose mutase from Aspergillus fumigatus.
van Straaten KE, Routier FH, Sanders DA
J Biol Chem 287 10780-90 (2012)
PMID: 22334662

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-galactopyranose mutase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 509 amino acids
Theoretical weight: 57.48 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4W1X2 (Residues: 2-510; Coverage: 100%)
Gene names: glf, glfA
Sequence domains: NAD(P)-binding Rossmann-like domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FDA 8 x FDA
2 bound ligands:
Ligand GDU 8 x GDU
Ligand CL 8 x CL
1 modified residue:
Ligand MSE 112 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 72.083Å b: 129.338Å c: 175.077Å
α: 89.97° β: 103.61° γ: 90.14°
R-values:
R R work R free
0.252 0.25 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Noncanonical reactions of flavoenzymes.
Sobrado P. (2012)
2 more

7 mentions without citation

Structure, mechanism, and dynamics of UDP-galactopyranose mutase.
Tanner et al. (2014)
6 more