3ulj

X-ray diffraction
1.06Å resolution

Crystal structure of apo Lin28B cold shock domain

Released:
Source organism: Xenopus tropicalis
Primary publication:
The Lin28 cold-shock domain remodels pre-let-7 microRNA.
Nucleic Acids Res 40 7492-506 (2012)
PMID: 22570413

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
PDBe Complex ID:
PDB-CPX-110084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein lin-28 homolog B Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.94 KDa
Source organism: Xenopus tropicalis
Expression system: Escherichia coli
UniProt:
  • Canonical: B4F6I0 (Residues: 27-114; Coverage: 35%)
Gene names: LIN-28, csdd2, lin28b
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 52.62Å b: 52.62Å c: 137.336Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.121 0.12 0.139
Expression system: Escherichia coli