› 3umb

X-ray diffraction
2.2Å resolution

Crystal Structure of the L-2-Haloacid Dehalogenase RSc1362

Released:
DOI: 10.2210/pdb3umb/pdb
PDB entry 3umb coloured by chain, front view.
PDB entry 3umb coloured by chain, side view.
PDB entry 3umb coloured by chain, top view.
Source organism: Ralstonia solanacearum
Entry authors: Petit P, Chan PWY, Savchenko A, Yakunin AF, Edwards EA, Pai EF

Function and Biology Details

Reaction catalysed: 
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-haloacid dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 25.95 KDa
Source organism: Ralstonia solanacearum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XZN3 (Residues: 1-233; Coverage: 100%)
Gene name: RSc1362
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand K 2 x K
Ligand CL 3 x CL
Ligand NA 1 x NA
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C222
Unit cell:
a: 66.38Å b: 129.57Å c: 54.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.283
Expression system: Escherichia coli BL21(DE3)

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