› 3umj

X-ray diffraction
2.1Å resolution

Crystal Structure of D311E Lipase

Released:
DOI: 10.2210/pdb3umj/pdb
PDB entry 3umj coloured by chain, front view.
PDB entry 3umj coloured by chain, side view.
PDB entry 3umj coloured by chain, top view.
Source organism: Geobacillus zalihae
Primary publication:
Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1.
Ruslan R, Abd Rahman RN, Leow TC, Ali MS, Basri M, Salleh AB
Int J Mol Sci 13 943-60 (2012)
PMID: 22312296

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 43.2 KDa
Source organism: Geobacillus zalihae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q842J9 (Residues: 30-416; Coverage: 93%)
Gene name: IC807_06045
Structure domains: alpha/beta hydrolase

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 3 x GOL
Ligand CL 2 x CL
Ligand NA 2 x NA
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 117.323Å b: 81.162Å c: 100.14Å
α: 90° β: 96.49° γ: 90°
R-values:
R R work R free
0.158 0.155 0.212
Expression system: Escherichia coli

Quick links

Citations

5 review citations

From structure to catalysis: recent developments in the biotechnological applications of lipases.
Anobom et al. (2014)
4 more

3 mentions without citation

Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1.
Ruslan et al. (2012)
2 more