› 3ums

X-ray diffraction
2.34Å resolution

Crystal structure of the G202A mutant of human G-alpha-i1

Released:
DOI: 10.2210/pdb3ums/pdb
PDB entry 3ums coloured by chain, front view.
PDB entry 3ums coloured by chain, side view.
PDB entry 3ums coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Correction for Regulators of G-protein Signaling accelerate GPCR signaling kinetics and govern sensitivity solely by accelerating GTPase activity
Lambert NA, Johnston CA, Cappell SD, Kuravi S, Kimple AJ, Willard FS, Siderovski DP
Proc. Natl. Acad. Sci. U.S.A. (2012)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4
Unit cell:
a: 121.531Å b: 121.531Å c: 67.909Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.228
Expression system: Escherichia coli BL21(DE3)

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