› 3une

X-ray diffraction
3.2Å resolution

Mouse constitutive 20S proteasome

Released:
DOI: 10.2210/pdb3une/pdb
PDB entry 3une coloured by chain, front view.
PDB entry 3une coloured by chain, side view.
PDB entry 3une coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Immuno- and constitutive proteasome crystal structures reveal differences in substrate and inhibitor specificity.
Huber EM, Basler M, Schwab R, Heinemeyer W, Kirk CJ, Groettrup M, Groll M
Cell 148 727-38 (2012)
PMID: 22341445

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126710 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit alpha type-2 Chains: A, O, c, q
Molecule details ›
Chains: A, O, c, q
Length: 234 amino acids
Theoretical weight: 25.96 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P49722 (Residues: 1-234; Coverage: 100%)
Gene names: Lmpc3, Psma2
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-4 Chains: B, P, d, r
Molecule details ›
Chains: B, P, d, r
Length: 261 amino acids
Theoretical weight: 29.51 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P0 (Residues: 1-261; Coverage: 100%)
Gene name: Psma4
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-7 Chains: C, Q, e, s
Molecule details ›
Chains: C, Q, e, s
Length: 248 amino acids
Theoretical weight: 27.9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9Z2U0 (Residues: 1-248; Coverage: 100%)
Gene name: Psma7
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-5 Chains: D, R, f, t
Molecule details ›
Chains: D, R, f, t
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9Z2U1 (Residues: 1-241; Coverage: 100%)
Gene name: Psma5
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-1 Chains: E, S, g, u
Molecule details ›
Chains: E, S, g, u
Length: 263 amino acids
Theoretical weight: 29.59 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P4 (Residues: 1-263; Coverage: 100%)
Gene name: Psma1
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-3 Chains: F, T, h, v
Molecule details ›
Chains: F, T, h, v
Length: 255 amino acids
Theoretical weight: 28.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O70435 (Residues: 1-255; Coverage: 100%)
Gene name: Psma3
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-6 Chains: G, U, i, w
Molecule details ›
Chains: G, U, i, w
Length: 246 amino acids
Theoretical weight: 27.41 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9QUM9 (Residues: 1-246; Coverage: 100%)
Gene name: Psma6
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-7 Chains: H, V, j, x
Molecule details ›
Chains: H, V, j, x
Length: 234 amino acids
Theoretical weight: 25.28 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P70195 (Residues: 44-277; Coverage: 85%)
Gene names: Mmc14, Psmb7
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-3 Chains: I, W, k, y
Molecule details ›
Chains: I, W, k, y
Length: 205 amino acids
Theoretical weight: 22.99 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P1 (Residues: 1-205; Coverage: 100%)
Gene name: Psmb3
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-2 Chains: J, X, l, z
Molecule details ›
Chains: J, X, l, z
Length: 201 amino acids
Theoretical weight: 22.94 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P3 (Residues: 1-201; Coverage: 100%)
Gene name: Psmb2
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-5 Chains: 1, K, Y, m
Molecule details ›
Chains: 1, K, Y, m
Length: 205 amino acids
Theoretical weight: 22.55 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O55234 (Residues: 60-264; Coverage: 78%)
Gene name: Psmb5
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-1 Chains: 2, L, Z, n
Molecule details ›
Chains: 2, L, Z, n
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O09061 (Residues: 28-240; Coverage: 89%)
Gene name: Psmb1
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-4 Chains: 3, M, a, o
Molecule details ›
Chains: 3, M, a, o
Length: 219 amino acids
Theoretical weight: 24.38 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P99026 (Residues: 46-264; Coverage: 83%)
Gene names: Lmp3, Psmb4
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-6 Chains: 4, N, b, p
Molecule details ›
Chains: 4, N, b, p
Length: 205 amino acids
Theoretical weight: 22.01 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q60692 (Residues: 34-238; Coverage: 86%)
Gene names: Lmp19, Psmb6
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 170.97Å b: 201.3Å c: 226.01Å
α: 90° β: 108.07° γ: 90°
R-values:
R R work R free
0.222 0.22 0.246

Quick links

Citations

84 review citations

Regulation of proteasome activity in health and disease.
Schmidt et al. (2014)
83 more

8 mentions without citation

Structural biology of the proteasome.
Kish-Trier et al. (2013)
7 more