› 3unf

X-ray diffraction
2.9Å resolution

Mouse 20S immunoproteasome in complex with PR-957

Released:
DOI: 10.2210/pdb3unf/pdb
PDB entry 3unf coloured by chain, front view.
PDB entry 3unf coloured by chain, side view.
PDB entry 3unf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Immuno- and constitutive proteasome crystal structures reveal differences in substrate and inhibitor specificity.
Huber EM, Basler M, Schwab R, Heinemeyer W, Kirk CJ, Groettrup M, Groll M
Cell 148 727-38 (2012)
PMID: 22341445

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126709 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit alpha type-2 Chains: A, O
Molecule details ›
Chains: A, O
Length: 234 amino acids
Theoretical weight: 25.96 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P49722 (Residues: 1-234; Coverage: 100%)
Gene names: Lmpc3, Psma2
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-4 Chains: B, P
Molecule details ›
Chains: B, P
Length: 261 amino acids
Theoretical weight: 29.51 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P0 (Residues: 1-261; Coverage: 100%)
Gene name: Psma4
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-7 Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 248 amino acids
Theoretical weight: 27.9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9Z2U0 (Residues: 1-248; Coverage: 100%)
Gene name: Psma7
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-5 Chains: D, R
Molecule details ›
Chains: D, R
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9Z2U1 (Residues: 1-241; Coverage: 100%)
Gene name: Psma5
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-1 Chains: E, S
Molecule details ›
Chains: E, S
Length: 263 amino acids
Theoretical weight: 29.59 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P4 (Residues: 1-263; Coverage: 100%)
Gene name: Psma1
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-3 Chains: F, T
Molecule details ›
Chains: F, T
Length: 255 amino acids
Theoretical weight: 28.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O70435 (Residues: 1-255; Coverage: 100%)
Gene name: Psma3
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-6 Chains: G, U
Molecule details ›
Chains: G, U
Length: 246 amino acids
Theoretical weight: 27.41 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9QUM9 (Residues: 1-246; Coverage: 100%)
Gene name: Psma6
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-10 Chains: H, V
Molecule details ›
Chains: H, V
Length: 234 amino acids
Theoretical weight: 24.83 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O35955 (Residues: 40-273; Coverage: 86%)
Gene names: Lmp10, Mecl1, Psmb10
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-3 Chains: I, W
Molecule details ›
Chains: I, W
Length: 205 amino acids
Theoretical weight: 22.99 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P1 (Residues: 1-205; Coverage: 100%)
Gene name: Psmb3
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-2 Chains: J, X
Molecule details ›
Chains: J, X
Length: 201 amino acids
Theoretical weight: 22.94 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P3 (Residues: 1-201; Coverage: 100%)
Gene name: Psmb2
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-8 Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 204 amino acids
Theoretical weight: 22.67 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P28063 (Residues: 73-276; Coverage: 74%)
Gene names: Lmp7, Mc13, Psmb8
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-1 Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O09061 (Residues: 28-240; Coverage: 89%)
Gene name: Psmb1
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-4 Chains: M, a
Molecule details ›
Chains: M, a
Length: 219 amino acids
Theoretical weight: 24.38 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P99026 (Residues: 46-264; Coverage: 83%)
Gene names: Lmp3, Psmb4
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-9 Chains: N, b
Molecule details ›
Chains: N, b
Length: 199 amino acids
Theoretical weight: 21.35 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P28076 (Residues: 21-219; Coverage: 91%)
Gene names: Lmp2, Psmb9, Ring12
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

4 bound ligands:
Ligand CL 41 x CL
Ligand K 13 x K
Ligand 04C 6 x 04C
Ligand IOD 6 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 117.3Å b: 194.6Å c: 157.7Å
α: 90° β: 107.1° γ: 90°
R-values:
R R work R free
0.237 0.235 0.275

Quick links

Citations

85 review citations

Regulation of proteasome activity in health and disease.
Schmidt et al. (2014)
84 more

12 mentions without citation

Structural biology of the proteasome.
Kish-Trier et al. (2013)
11 more