› 3uof

X-ray diffraction
2.9Å resolution

Mycobacterium tuberculosis bacterioferritin, BfrA

Released:
DOI: 10.2210/pdb3uof/pdb
PDB entry 3uof coloured by chain, front view.
PDB entry 3uof coloured by chain, side view.
PDB entry 3uof coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: McMath LM, Goulding CW, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin BfrA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 161 amino acids
Theoretical weight: 18.55 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPQ9 (Residues: 1-159; Coverage: 100%)
Gene names: MTCY180.42c, Rv1876, bfr, bfrA
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:
Ligand FE 12 x FE
Ligand TRS 3 x TRS
Ligand HEM 3 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I4
Unit cell:
a: 122.797Å b: 122.797Å c: 174.691Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.208 0.298
Expression system: Escherichia coli BL21(DE3)

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