› 3upy

X-ray diffraction
1.8Å resolution

Crystal structure of the Brucella abortus enzyme catalyzing the first committed step of the methylerythritol 4-phosphate pathway.

Released:
DOI: 10.2210/pdb3upy/pdb
Model geometry
Fit model/data
PDB entry 3upy coloured by chain, front view.
PDB entry 3upy coloured by chain, side view.
PDB entry 3upy coloured by chain, top view.
Source organism: Brucella abortus 2308
Primary publication:
Crystal structure of Brucella abortus deoxyxylulose-5-phosphate reductoisomerase-like (DRL) enzyme involved in isoprenoid biosynthesis.
Pérez-Gil J, Calisto BM, Behrendt C, Kurz T, Fita I, Rodríguez-Concepción M
J Biol Chem 287 15803-9 (2012)
PMID: 22442144

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAF domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 47.66 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YIM3 (Residues: 1-437; Coverage: 100%)
Gene name: BAB2_0264
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand FOM 1 x FOM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 49.391Å b: 63.379Å c: 79.334Å
α: 68.79° β: 75.54° γ: 72.07°
R-values:
R R work R free
0.165 0.163 0.204
Expression system: Escherichia coli

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Citations

1 review citation

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
2 other articles

2 mentions without citation

Crystal structure of Brucella abortus deoxyxylulose-5-phosphate reductoisomerase-like (DRL) enzyme involved in isoprenoid biosynthesis.
Pérez-Gil et al. (2012)
1 more