› 3uq7

X-ray diffraction
3.8Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240S F247L (L9S F16L) in presence of 10 mM cysteamine

Released:
DOI: 10.2210/pdb3uq7/pdb
PDB entry 3uq7 coloured by chain, front view.
PDB entry 3uq7 coloured by chain, side view.
PDB entry 3uq7 coloured by chain, top view.
Source organism: Dickeya dadantii 3937
Primary publication:
Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals.
Gonzalez-Gutierrez G, Lukk T, Agarwal V, Papke D, Nair SK, Grosman C
Proc Natl Acad Sci U S A 109 6331-6 (2012)
PMID: 22474383

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 324 amino acids
Theoretical weight: 36.96 KDa
Source organism: Dickeya dadantii 3937
Expression system: Escherichia coli
UniProt:
  • Canonical: E0SJQ4 (Residues: 22-343; Coverage: 100%)
Gene name: Dda3937_00520
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 105.38Å b: 266.45Å c: 110.91Å
α: 90° β: 109.47° γ: 90°
R-values:
R R work R free
0.216 0.215 0.244
Expression system: Escherichia coli

Quick links

Citations

8 review citations

A gating mechanism of pentameric ligand-gated ion channels.
Calimet et al. (2013)
7 more

5 mentions without citation

Structure and Pharmacologic Modulation of Inhibitory Glycine Receptors.
Burgos et al. (2016)
4 more