› 3uqe

X-ray diffraction
2.2Å resolution

Crystal structure of the Phosphofructokinase-2 mutant Y23D from Escherichia coli

Released:
DOI: 10.2210/pdb3uqe/pdb
PDB entry 3uqe coloured by chain, front view.
PDB entry 3uqe coloured by chain, side view.
PDB entry 3uqe coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Pereira HM, Caniuguir A, Baez M, Cabrera R, Garatt RC, Babul J

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase isozyme 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 32.44 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06999 (Residues: 1-309; Coverage: 100%)
Gene names: JW5280, b1723, pfkB
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

3 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 4 x MG
Ligand POP 2 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P2221
Unit cell:
a: 43.838Å b: 89.123Å c: 175.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.253
Expression system: Escherichia coli BL21(DE3)

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