› 3ur1

X-ray diffraction
4.5Å resolution

The structure of a ternary complex between CheA domains P4 and P5 with CheW and with a truncated fragment of TM14, a chemoreceptor analog from Thermotoga maritima.

Released:
DOI: 10.2210/pdb3ur1/pdb
PDB entry 3ur1 coloured by chain, front view.
PDB entry 3ur1 coloured by chain, side view.
PDB entry 3ur1 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
The structure of native bacterial chemoreceptor arrays
Breigel A, Li X, Bilwes AM, Hugues KT, Jensen GJ, Crane BR
Proc. Natl. Acad. Sci. U.S.A. (2012)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-176350 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chemotaxis protein CheA Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 35.55 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56310 (Residues: 355-671; Coverage: 47%)
Gene names: TM_0702, cheA
Sequence domains:
Chemotaxis protein CheW Chain: B
Molecule details ›
Chain: B
Length: 139 amino acids
Theoretical weight: 15.72 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56311 (Residues: 9-147; Coverage: 92%)
Gene names: TM_0701, cheW
Sequence domains: CheW-like domain
Methyl-accepting transducer domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 85 amino acids
Theoretical weight: 9.45 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7DFA3 (Residues: 107-191; Coverage: 31%)
Gene name: TM_0014
Sequence domains: Methyl-accepting chemotaxis protein (MCP) signalling domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: R32
Unit cell:
a: 213.991Å b: 213.991Å c: 208.192Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.27 0.266 0.291
Expression system: Escherichia coli

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