› 3usg

X-ray diffraction
2.5Å resolution

Crystal structure of LeuT bound to L-leucine in space group C2 from lipid bicelles

Released:
DOI: 10.2210/pdb3usg/pdb
PDB entry 3usg coloured by chain, front view.
PDB entry 3usg coloured by chain, side view.
PDB entry 3usg coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context.
Wang H, Elferich J, Gouaux E
Nat Struct Mol Biol 19 212-9 (2012)
PMID: 22245965

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58.08 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

6 bound ligands:
Ligand LEU 1 x LEU
Ligand NA 2 x NA
Ligand ACT 1 x ACT
Ligand PEG 1 x PEG
Ligand UND 1 x UND
Ligand PC 1 x PC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 123.814Å b: 90.859Å c: 81.645Å
α: 90° β: 103.78° γ: 90°
R-values:
R R work R free
0.214 0.213 0.231
Expression system: Escherichia coli

Quick links

Citations

18 review citations

The magic of bicelles lights up membrane protein structure.
Dürr et al. (2012)
17 more

3 mentions without citation

Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context.
Wang et al. (2012)
2 more